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Journal / Biochemistry / 在质膜用新型荧光探针磷脂酰-4,5-二磷酸的单分子超分辨率成像…
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Biochemistry

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JoVE Journal Biochemistry
在质膜用新型荧光探针磷脂酰-4,5-二磷酸的单分子超分辨率成像
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在质膜用新型荧光探针磷脂酰-4,5-二磷酸的单分子超分辨率成像

Article DOI: 10.3791/54466-v • 07:26 min • October 15th, 2016
October 15th, 2016 •
1, 1
1Department of Neuroscience, University of Wisconsin-Madison

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PI（4,5）P2调节各种细胞功能，但其在细胞质膜的纳米级组织知之甚少。由标记的PI（4,5）P 2与融合于普列克底物蛋白同源结构域的双色荧光探针，我们描述了一种新的方法来研究的PI（4,5）P2在质膜2的空间分布在纳米尺度。

Tags

生物化学，第116，超分辨率成像，PALM，磷脂酰肌醇4,5-二磷酸（PI（4,5）P2）细胞膜片，磷酸肌醇，β细胞
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