JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
单分子水平标记均匀性的全蛋白质定量
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Please note that all translations are automatically generated.
Click here for the English version.
单分子水平标记均匀性的全蛋白质定量
DOI:
10.3791/59199-v
•
08:29 min
•
April 19, 2019
•
Simon Leclerc
2
,
Youri Arntz
,
Yuichi Taniguchi
3
1
Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research
,
RIKEN
,
2
Laboratoire de Biomatériaux et Bioimagerie
,
INSERM
,
3
PRESTO, Japan Science and Technology Agency
Chapters
00:04
Title
01:33
Cell Lysis and Fluorescent Labeling
03:26
Proteome Separation and Recovery
03:51
Microscope Coverslip Preparation
05:46
Observation with Single Molecule Fluorescence Microscopy
06:07
Results: Labeling Homogeneity at the Single Molecule Level
07:33
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们提出了一个协议, 以评估标签同质性的每个蛋白质物种在一个复杂的蛋白质样本在单分子水平。
Tags
Protein Quantification
Single-molecule Analysis
Fluorescent Labeling
Heterogeneity
Molecular Concentration Assays
Diagnostic Methods
Protein Species Analysis
Data Reproducibility
Lysing Buffer
Tween-20
DTT
CHAPS
Cy3 NHS Ester Dye
HEPES-NaOH
Biotin-2-amine
EDC
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
增强组织样品使用组合前体同位素标记和标记等压的复用(cPILOT)
单分子水平的高精度FRET用于生物分子结构测定
磁性镊子 quadruplexes 单分子操作
应用三色单分子焦虑研究蛋白质相互作用的相关性
单分子力谱中蛋白质共价固定化的研究
单分子跟踪显微镜 - 确定细胞分子扩散状态的工具
使用新激酶蛋白的强生化方法在分子水平上进行表征
用于分析依赖帽的翻译动力学的
体外
单分子成像测定
单分子鱼的转录活性等位基因单细胞分析
限制性核酸内切酶介导的 DNA 切割的单分子停留时间分析
Read Article