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Journal
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Biochemistry
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生产用于单分子观察的DNA折纸纳米结构的Dynein和Kinesin电机组合
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Please note that all translations are automatically generated.
Click here for the English version.
生产用于单分子观察的DNA折纸纳米结构的Dynein和Kinesin电机组合
DOI:
10.3791/60369-v
•
08:09 min
•
October 15, 2019
•
Jingjie Hu
,
Nathan D. Derr
2
1
Department of Biological Sciences
,
Smith College
,
2
Center for Microscopy and Imaging
,
Smith College
Chapters
00:04
Title
00:46
Galactose-Induced Motor Protein Growth, Expression, and Harvesting
01:39
Cell Lysis and Soluble Protein Extraction
02:36
IgG Affinity Purification
03:17
DNA Oligonucleotide Labeling and TEV Cleavage
04:50
Chassis Purification
05:56
Results: Representative Motor Ensemble Production
07:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议的目标是在DNA折纸纳米结构上形成分子马达组合,并使用全内反射荧光显微镜观察整体运动。
Tags
DNA Origami
Cytoskeletal Motor Proteins
Dynein
Kinesin
Myosin
Cargo Transport
Single Molecule Observation
Yeast Expression
Protein Purification
IgG Affinity Purification
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