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生产用于单分子观察的DNA折纸纳米结构的Dynein和Kinesin电机组合
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
DOI:

08:09 min

October 15, 2019

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Chapters

  • 00:04Title
  • 00:46Galactose-Induced Motor Protein Growth, Expression, and Harvesting
  • 01:39Cell Lysis and Soluble Protein Extraction
  • 02:36IgG Affinity Purification
  • 03:17DNA Oligonucleotide Labeling and TEV Cleavage
  • 04:50Chassis Purification
  • 05:56Results: Representative Motor Ensemble Production
  • 07:26Conclusion

Summary

Automatic Translation

该协议的目标是在DNA折纸纳米结构上形成分子马达组合,并使用全内反射荧光显微镜观察整体运动。

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