Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Método para la identificación de inhibidores de…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Método para la identificación de inhibidores de moléculas pequeñas de la interacción proteína-proteína entre HCN1 y TRIP8b
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Journal (Biochemistry)
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Journal (Biochemistry)
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
 
Click here for the English version

Método para la identificación de inhibidores de moléculas pequeñas de la interacción proteína-proteína entre HCN1 y TRIP8b

Article DOI: 10.3791/54540-v • 10:20 min • November 11th, 2016
November 11th, 2016 •
*1, *1, 2, 3, 1, 1, 4, 1,5
1Davee Department of Neurology and Clinical Neurosciences, Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery, Northwestern University, 3Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences, Northwestern University, 5Department of Physiology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

La interacción entre los canales HCN y su subunidad auxiliar ha sido identificado como un objetivo terapéutico en el trastorno depresivo mayor. Aquí, un método basado en la polarización de fluorescencia para identificar inhibidores de molécula pequeña de esta interacción proteína-proteína, se presenta.

Tags

Bioquímica Número 117 el cribado de alto rendimiento descubrimiento de fármacos interacciones proteína-proteína la polarización de fluorescencia HCN TRIP8b
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter