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Journal
/
Biochemistry
/
方法确定HCN1和TRIP8b之间蛋白 - 蛋白相互作用的小分子抑制剂
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
Please note that all translations are automatically generated.
Click here for the English version.
方法确定HCN1和TRIP8b之间蛋白 - 蛋白相互作用的小分子抑制剂
DOI:
10.3791/54540-v
•
10:20 min
•
November 11, 2016
•
Ye Han*
1
,
Kyle A. Lyman*
1
,
Matt Clutter
,
Gary E. Schiltz
,
Quratul-Ain Ismail
,
Xiangying Cheng
,
Chi-Hao Luan
,
Dane M. Chetkovich
5
1
Davee Department of Neurology and Clinical Neurosciences
,
Feinberg School of Medicine, Northwestern University
,
2
Center for Molecular Innovation and Drug Discovery
,
Northwestern University
,
3
Department of Pharmacology
,
Feinberg School of Medicine, Northwestern University
,
4
High Throughput Analysis Laboratory, Department of Molecular Biosciences
,
Northwestern University
,
5
Department of Physiology
,
Feinberg School of Medicine, Northwestern University
Chapters
00:05
Title
01:08
High Throughput Screen
03:07
Confirmation of Hits Using TAMRA-tagged HCN1 Peptide
04:33
Dose-response Validation of Bead-based Proximity Assay
07:15
Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions
09:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
HCN通道和其辅助亚基之间的相互作用已被确定为在抑郁症的治疗靶点。这里,用于识别这种蛋白质 - 蛋白质相互作用的小分子抑制剂荧光基于偏振的方法,提出。
Tags
Small Molecule Inhibitors
Protein-protein Interaction
HCN1
TRIP8b
High-throughput Screening
Fluorescence Polarization
Inhibition Assay
Depression
Therapeutic Target
Acoustic Liquid Handler
384-well Plate
FITC-labeled HCN1
TAMRA-labeled HCN1
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