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Journal / Medicine / Generazione rapida ed efficace delle ricombinante …
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Generazione rapida ed efficace delle ricombinante cellule staminali umane pluripotenti da ricombinasi mediata scambio Cassette in
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Generazione rapida ed efficace delle ricombinante cellule staminali umane pluripotenti da ricombinasi mediata scambio Cassette in

Article DOI: 10.3791/54718-v • 11:36 min • November 20th, 2016
November 20th, 2016 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Stem Cell Institute, Katholieke Universiteit Leuven
* These authors contributed equally

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Qui riportiamo un metodo di modifica genetica rapida ed efficiente in base RMCE nel locus AAVS1 di umani cellule staminali pluripotenti (hPSCs) che migliora i sistemi precedentemente descritti. Usando questa tecnica, linee isogeniche possono essere rapidamente e affidabile generati per adeguati studi comparativi, facilitando la ricerca transgenesi mediata con hPSCs.

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Medicina cellule staminali pluripotenti umane ingegneria genomica ricombinasi mediata scambio di cassette, flippase ricombinasi porto sicuro.
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