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Hurtig og effektiv Generation af rekombinant human pluripotente stamceller ved rekombinase-medieret Cassette Exchange i
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Hurtig og effektiv Generation af rekombinant human pluripotente stamceller ved rekombinase-medieret Cassette Exchange i

Article DOI: 10.3791/54718-v • 11:36 min • November 20th, 2016
November 20th, 2016 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Stem Cell Institute, Katholieke Universiteit Leuven
* These authors contributed equally

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Her rapporterer vi en hurtig og effektiv gen redigering metode baseret på RMCE i AAVS1 locus af humane pluripotente stamceller (hPSCs), der forbedrer tidligere beskrevne systemer. Ved hjælp af denne teknik, kan isogene linjer hurtigt og pålideligt genereret for ordentlig sammenlignende undersøgelser, lette transgenese-medieret forskning med hPSCs.

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Medicin menneskelige pluripotente stamceller genomisk teknik rekombinase medieret kassette udveksling, Flippase rekombinase sikker havn.
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