Journal
/
Medicine
/
Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

DOI:
10.3791/57401-v

•

09:03 min

•

June 19, 2018

•

, , , , , , ,
1Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research

Chapters

  • 00:05Title
  • 01:03OT-I T Cell Staining and Adoptive Transfer
  • 04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
  • 05:21Flow Cytometry
  • 07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.

Tags

OT1 CD8 T CellsCFSEAdoptive TransferAdjuvantCytotoxic T Lymphocyte (CTL) ResponseVaccine DevelopmentLymph NodeSpleenMagnetic Bead Isolation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Teratoma Generation in the Testis Capsule

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

In vivo 19F MRI for Cell Tracking

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Read Article