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JoVE Journal
Medicine

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通过重组酶介导的盒式交换在重组人多能干细胞的快速高效生成
 
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通过重组酶介导的盒式交换在重组人多能干细胞的快速高效生成

Article DOI: 10.3791/54718-v 11:36 min November 20th, 2016
November 20th, 2016

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Summary

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Click here for the English version.

这里,我们报告在先前所描述的系统,提高了在人多能干细胞(hPSCs)的AAVS1轨迹基于RMCE迅速和有效的基因编辑方法。使用这种技术,基因系可迅速而可靠地进行适当的比较研究产生,促进与hPSCs转基因介导的研究。

Tags

医药,第117,人多能干细胞,基因组工程,重组酶介导的盒式交换,
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