Understanding the cellular and molecular mechanisms of re-endothelialization following arterial denudation injury is of paramount importance in preventing thrombosis and restenosis of arteries. Here we describe a protocol for reproducible arterial denudation injury of the infrarenal abdominal aorta. The procedure was developed to investigate the underlying mechanisms that regulate endothelial regeneration using mouse models.
Shirali, A. S., McDonald, A. I., Mack, J. J., Iruela-Arispe, M. L. Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model. J. Vis. Exp. (117), e54755, doi:10.3791/54755 (2016).