Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model

Aditya S. Shirali; Austin I. McDonald; Julia J. Mack; M. Luisa Iruela-Arispe

doi:10.3791/54755

Journal

/

Medicine

/

Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model

9,242 Views

•

08:33 min

•

November 24, 2016

DOI:

10.3791/54755-v

DOI:

10.3791/54755-v

•

08:33 min

November 24, 2016

•

9226 Views

Aditya S. Shirali¹, Austin I. McDonald², Julia J. Mack³, M. Luisa Iruela-Arispe³

¹Department of Surgery,University of California, Los Angeles ²Molecular Biology Institute,University of California, Los Angeles ³Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles

Summary

Understanding the cellular and molecular mechanisms of re-endothelialization following arterial denudation injury is of paramount importance in preventing thrombosis and restenosis of arteries. Here we describe a protocol for reproducible arterial denudation injury of the infrarenal abdominal aorta. The procedure was developed to investigate the underlying mechanisms that regulate endothelial regeneration using mouse models.