Name: Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia (Video) | JoVE Journal
Uploaded: 2017-07-22T13:00:00.000+00:00
Duration: 8 min 10 s
Description: Learn a minimally invasive technique to create a reproducible spinal cord ischemia model in rats using Fogarty catheter occlusion. This method enables controlled study of hind limb motor deficits for neuroscience research.

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The authors have no acknowledgements.

This protocol was approved by the Institutional Animal Care and Use Committee of Seoul National University Bundang Hospital. Animal care and experiments were conducted according to the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals.
1. Surgical Preparation
<ol>
	<li>Prior to surgery, flush the catheters with sterile saline to ensure patency.</li>
	<li>Put a heating blanket on the operating table, and cover the table with a sterile drape.</li>
	<li>Place Male Sprague&#8722;Dawley rats (270-330 g) in an acrylic box with 3.0%-4.0% of isoflurane in 100% oxygen.</li>
	<li>Apply lubricant to the rat&#39;s eyes.</li>
	<li>Place the rat on the operating table in the supine position, and maintain anesthesia using a facial mask with continuous administration of inhaled isoflurane (1.0%-2.5 vol.%).</li>
	<li>Place a rectal probe to monitor and maintain body temperature between 37.0-38.0 &#176;C.</li>
</ol>
2. Femoral Artery Catheterization
<ol>
	<li>Gently scrub the right inguinal area using Betadine and 70% ethanol.</li>
	<li>Make a 2-cm horizontal skin incision using scissors on the right inguinal area.</li>
	<li>Using a retractor, expose the surgical field.</li>
	<li>Dissect the femoral artery from the surrounding vein and nerve. Isolate a 1-cm section of the artery using curved forceps and blunted forceps.</li>
	<li>Using a 4.0 black silk suture, place a loose tie on both the proximal and distal ends of the artery to maximize the exposure.</li>
	<li>Make an incision on the femoral artery using micro-scissors.</li>
	<li>Insert a 2-Fr. balloon-tipped catheter into the femoral artery using micro-forceps. Secure the catheter to the vessel about 1 cm from the catheter head with the proximal ligature, and then tie the distal ligature.</li>
</ol>
3. Carotid Artery Catheterization
<ol>
	<li>Scrub the right anterior neck using betadine and 70% ethanol, and then make a skin incision.</li>
	<li>Using a retractor, expose the surgical field. Isolate a 1-cm section of the artery, and tie it loosely using a silk suture on both the proximal and distal ends of the artery to maximize the exposure.</li>
	<li>Puncture the carotid artery using a 24 G intravenous catheter, and advance the proximal 1 cm of the catheter towards the heart.</li>
	<li>Secure the catheter with the proximal ligature, and then tie the distal ligature.</li>
	<li>Using a 3-way stopcock, connect the distal end of the catheter to the saline-filled micro-tube and external reservoir.</li>
</ol>
4. Tail Artery Catheterization
<ol>
	<li>Scrub the ventral area of the tail using betadine and 70% ethanol, and make a 2-cm skin incision.</li>
	<li>Dissect the tail artery from the surrounding structures and isolate a 1 cm section of the artery using curved forceps and blunted forceps.</li>
	<li>Using a 4.0 black silk suture, place a loose tie on both the proximal and distal ends of the artery to maximize the exposure.</li>
	<li>Puncture the tail artery using a 24 G intravenous catheter, and advance the catheter into the artery.</li>
	<li>Secure the catheter to the vessel (about 1 cm from the catheter head) with the proximal ligature, and tie the distal ligature.</li>
	<li>Connect the distal part of the catheter to the arterial pressure monitoring device.</li>
</ol>
5. Induction of Spinal Cord Ischemia
<ol>
	<li>After catheterization is complete, advance the 2 Fr. balloon-tipped catheter into the descending thoracic aorta so that the tip of the catheter reaches the left subclavian artery.</li>
	<li>Insert to a depth of 10 cm from the insertion site.</li>
	<li>Inject 150 units of heparin at a concentration of 100 units/mL into the carotid catheter.</li>
	<li>Inflate the catheter balloon with 0.05 mL of saline.</li>
	<li>Simultaneously, drain the blood into the external blood container from the carotid artery to regulate the proximal arterial pressure to 80 mmHg.</li>
	<li>Connect the arterial pressure monitoring system to the remaining lumen of the 3-way stop cock, and control the amount of blood drainage with monitoring the arterial pressure.</li>
	<li>Confirm the success of aortic occlusion by an abrupt decrease and continuous loss of distal arterial pressure.</li>
	<li>After aortic occlusion of 9, 10, or 11 min, deflate the Fogarty catheter balloon, and reinfuse the drained blood.</li>
</ol>
6. Post-surgical Care and Neurologic Assessment
<ol>
	<li>While monitoring the arterial pressure through the tail artery, remove the catheters from the femoral and carotid arteries and close the wounds with silk sutures.</li>
	<li>After confirming that the arterial pressure is recovered to the normal range, remove the catheter from the tail artery and close the wound.</li>
	<li>After recovery from the anesthesia, return the rat to its cage.</li>
	<li>Assess hind limb motor function at 24 h after surgery using the motor deficit index (Table 1). The motor deficit index is defined as the sum of the ambulation score and the placing/stepping reflex score. A motor deficit index of 6 indicates the maximum deficit.</li>
</ol>
7. Histopathologic Evaluation
<ol>
	<li>After the neurologic assessment, anesthetize rats with mask-delivered isoflurane, sacrifice them by transcardial perfusion with 100 mL of heparinized saline under anesthesia, and extract the spinal cord.8</li>
	<li>Embed the cord sections from the level of lumbar vertebrae 3-5 in paraffin.</li>
	<li>Stain transverse sections with hematoxylin and eosin (H &#38; E).</li>
	<li>Observe the motor neuron injury under a microscope.9</li>
</ol>
8. Statistical Analysis
<ol>
	<li>Perform statistical analysis using Statistical Package for the Social Sciences version 20. The motor deficit index of the three groups was compared using the Kruskal-Wallis test, followed by a Mann-Whitney U-test. A P-value &#60;0.05 was considered statistically significant.</li>
</ol>

<ol><li>Greenberg, R. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Contemporary+analysis+of+descending+thoracic+and+thoracoabdominal+aneurysm+repair%3A+a+comparison+of+endovascular+and+open+techniques%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Contemporary analysis of descending thoracic and thoracoabdominal aneurysm repair: a comparison of endovascular and open techniques.</a> Circulation. 118 (8), 808-817 (2008).</li>
<li>Okita, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fighting+spinal+cord+complication+during+surgery+for+thoracoabdominal+aortic+disease%5BTitle%5D)+AND+%22Gen+Thorac+Cardiovasc+Surg%22%5BJournal%5D)">Fighting spinal cord complication during surgery for thoracoabdominal aortic disease.</a> Gen Thorac Cardiovasc Surg. 59 (2), 79-90 (2011).</li>
<li>Fleck, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+outcome+in+thoracoabdominal+aortic+aneurysm+repair%3A+the+role+of+cerebrospinal+fluid+drainage%5BTitle%5D)+AND+%22Neurocrit+Care%22%5BJournal%5D)">Improved outcome in thoracoabdominal aortic aneurysm repair: the role of cerebrospinal fluid drainage.</a> Neurocrit Care. 2 (1), 11-16 (2005).</li>
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<li>Taira, Y., Marsala, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+proximal+arterial+perfusion+pressure+on+function%2C+spinal+cord+blood+flow%2C+and+histopathologic+changes+after+increasing+intervals+of+aortic+occlusion+in+the+rat%5BTitle%5D)+AND+%22Stroke%22%5BJournal%5D)">Effect of proximal arterial perfusion pressure on function, spinal cord blood flow, and histopathologic changes after increasing intervals of aortic occlusion in the rat.</a> Stroke. 27 (10), 1850-1858 (1996).</li>
<li>Tveten, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spinal+cord+vascularity.+III.+The+spinal+cord+arteries+in+man%5BTitle%5D)+AND+%22Acta+Radiol+Diagn+%28Stockh%29%22%5BJournal%5D)">Spinal cord vascularity. III. The spinal cord arteries in man.</a> Acta Radiol Diagn (Stockh). 17 (3), 257-273 (1976).</li>
<li>Woollam, D. H., Millen, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+arterial+supply+of+the+spinal+cord+and+its+significance%5BTitle%5D)+AND+%22J+Neurol+Neurosurg+Psychiatry%22%5BJournal%5D)">The arterial supply of the spinal cord and its significance.</a> J Neurol Neurosurg Psychiatry. 18 (2), 97-102 (1955).</li>
<li>Kennedy, H. S., Puth, F., Van Hoy, M., Le Pichon, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+method+for+removing+the+brain+and+spinal+cord+as+one+unit+from+adult+mice+and+rats%5BTitle%5D)+AND+%22Lab+Anim+%28NY%29%22%5BJournal%5D)">A method for removing the brain and spinal cord as one unit from adult mice and rats.</a> Lab Anim (NY). 40 (2), 53-57 (2011).</li>
<li>Umehara, S., Goyagi, T., Nishikawa, T., Tobe, Y., Masaki, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Esmolol+and+landiolol%2C+selective+%CE%B21+adrenoreceptor+antagonists%2C+provide+neuroprotection+against+spinal+cord+ischemia+and+reperfusion+in+rats%5BTitle%5D)+AND+%22Anesth+Analg%22%5BJournal%5D)">Esmolol and landiolol, selective β1 adrenoreceptor antagonists, provide neuroprotection against spinal cord ischemia and reperfusion in rats.</a> Anesth Analg. 110 (4), 1133-1137 (2010).</li>
<li>De Ley, G., Nshimyumuremyi, J. B., Leusen, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hemispheric+blood+flow+in+the+rat+after+unilateral+common+carotid+occlusion%3A+evolution+with+time%5BTitle%5D)+AND+%22Stroke%22%5BJournal%5D)">Hemispheric blood flow in the rat after unilateral common carotid occlusion: evolution with time.</a> Stroke. 16 (1), 69-73 (1985).</li>
<li>Coyle, P., Panzenbeck, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Collateral+development+after+carotid+artery+occlusion+in+Fischer+344+rats%5BTitle%5D)+AND+%22Stroke%22%5BJournal%5D)">Collateral development after carotid artery occlusion in Fischer 344 rats.</a> Stroke. 21 (2), 316-321 (1990).</li>
<li>Levine, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anoxic-ischemic+encephalopathy+in+rats%5BTitle%5D)+AND+%22Am+J+Pathol%22%5BJournal%5D)">Anoxic-ischemic encephalopathy in rats.</a> Am J Pathol. 36, 1-17 (1960).</li>
<li>Prior, B. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Time+course+of+changes+in+collateral+blood+flow+and+isolated+vessel+size+and+gene+expression+after+femoral+artery+occlusion+in+rats%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Time course of changes in collateral blood flow and isolated vessel size and gene expression after femoral artery occlusion in rats.</a> Am J Physiol Heart Circ Physiol. 287 (6), H2434-H2447 (2004).</li>
<li>Yang, H. T., Feng, Y., Allen, L. A., Protter, A., Terjung, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficacy+and+specificity+of+bFGFincreased+collateral+flow+in+experimental+peripheral+arterial+insufficiency%5BTitle%5D)+AND+%22Am+J+Physiol+Heart+Circ+Physiol%22%5BJournal%5D)">Efficacy and specificity of bFGFincreased collateral flow in experimental peripheral arterial insufficiency.</a> Am J Physiol Heart Circ Physiol. 278 (6), H1966-H1973 (2000).</li>
<li>Kakinohana, M., Fuchigami, T., Nakamura, S., Sasara, T., Kawabata, T., Sugahara, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intrathecal+administration+of+morphine%2C+but+not+small+dose%2C+induced+spastic+paraparesis+after+a+noninjurious+interval+of+aortic+occlusion+in+rats%5BTitle%5D)+AND+%22Anesth+Analg%22%5BJournal%5D)">Intrathecal administration of morphine, but not small dose, induced spastic paraparesis after a noninjurious interval of aortic occlusion in rats.</a> Anesth Analg. 96 (3), 769-775 (2003).</li>
<li>Horiuchi, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+effects+of+the+delta-opioid+agonist+SNC80+on+hind-limb+motor+function+and+neuronal+injury+after+spinal+cord+ischemia+in+rats%5BTitle%5D)+AND+%22Anesth+Analg%22%5BJournal%5D)">The effects of the delta-opioid agonist SNC80 on hind-limb motor function and neuronal injury after spinal cord ischemia in rats.</a> Anesth Analg. 99 (1), 235-240 (2004).</li>
<li>Griepp, R. B., Griepp, E. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spinal+cord+perfusion+and+protection+during+descending+thoracic+and+thoracoabdominal+aortic+surgery%3A+the+collateral+network+concept%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Spinal cord perfusion and protection during descending thoracic and thoracoabdominal aortic surgery: the collateral network concept.</a> Ann Thorac Surg. 83 (2), S865-S869 (2007).</li>
</ol>

The authors have no competing financial interests. This work was supported by grant 2012R1A1A3014010 from the National Research Foundation of Korean Government.

In the current study, we demonstrated a rat model of spinal cord ischemia based on Taira and Marsala&#39;s method5 that induces variable degrees of motor deficit in the hind limb depending on the aortic occlusion time.
The length of aortic occlusion can affect the degree of motor deficit. If the aortic occlusion time is longer, the motor deficit becomes more severe. Thus, researchers can achieve a certain degree of motor deficit by controlling the aortic occlusion time ...

Paraplegia is a fatal complication of thoracoabdominal aortic aneurysm surgery. It results from spinal cord ischemia-reperfusion injury that occurs during cross-clamping and unclamping of the aorta.1 Several strategies including systemic hypothermia and cerebrospinal drainage have been introduced to protect the spinal cord,2,3,4 but many patients remain affected by the injury.
Several animal spinal cord ischemia models have been introduced to investigate its pathogenesis and devise protective strategies against the injury. In the current study, we outline a rat model of spinal cord ischemia based on Taira and Marsala&#39;s method.5 The spinal circulation system in rats is very similar to the spinal cord vascular and collateral system in humans, although there are some differences in the size and location.6,7 Thus, a rat is an anatomically suitable animal to utilize for an experimental model investigating the pathogenesis, complications, and treatment of spinal cord ischemia. Moreover, this spinal cord ischemia model produces reliable aortic occlusion with minimal intervention by utilizing an intravascular balloon occlusion of the thoracic aorta.
In this study, we demonstrated that this rat model of spinal cord ischemia induces reproducible motor deficits in the hind limbs that vary in severity depending on the aortic occlusion time.

<table><tbody><tr><td>Fogarty Arterial Embolectomy catheter</td><td>Edward Life Sciences</td><td>120602F</td><td>a balloon-tipped catheter inserted into the femoral artery</td></tr><tr><td>BD Insyte-N Autoguard Shielded IV catheter&amp;nbsp;</td><td>BD&amp;nbsp;</td><td>381411</td><td>24-gauge intravenous catheter</td></tr><tr><td>50 mL syringe</td><td>KOREA VACCINE&amp;nbsp;</td><td>KOVAX-SYRINGE 50mL</td><td>Facial mask</td></tr><tr><td>1 mL syringe</td><td>KOREA VACCINE</td><td>KOVAX-SYRINGE 1ml</td><td></td></tr><tr><td>Recal probe</td><td>HARVARD APPARATUS</td><td>50-7221F</td><td>Rectal probe for temperature monitoring</td></tr><tr><td>Micro dissecting spring scissor</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>JD-S-10</td><td>Micro-scissor</td></tr><tr><td>SCISSOR (SHARP-SHARP)</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>S-51-12-S</td><td>Scissors</td></tr><tr><td>Retractor</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>JD-S-74A</td><td>Retractor</td></tr><tr><td>Micro forcep&amp;nbsp;</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>JD-S-29</td><td>Micro-forceps</td></tr><tr><td>MOSQUITO FORCEP (Curved)</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>S-44-CPK</td><td>Curved forceps</td></tr><tr><td>DRESSING FORCEP&amp;nbsp;</td><td>Jeung do bio &amp;amp; Plant co.LTD.</td><td>S-37-16S</td><td>Blunted forceps</td></tr><tr><td>4/0 black silk&amp;nbsp;</td><td>Woori Medical</td><td>S431</td><td>4.0 black silk suture</td></tr><tr><td>3-WAY STOCK</td><td>Seonwon Medcal</td><td>D-98-01</td><td>3-way stopcock</td></tr><tr><td>Patient monitor</td><td>PHILIPS</td><td>MP20</td><td>The arterial pressure monitoring device.&amp;nbsp;</td></tr><tr><td>Heating blanket</td><td>Self production</td><td></td><td>Heating blanket</td></tr><tr><td>Microtube and external reservoir</td><td>Self production</td><td></td><td>Microtube and external reservoir</td></tr><tr><td>Heparin</td><td>JW Pharmaceutical</td><td></td><td>Heparin</td></tr><tr><td>0.9% NS 1000ml</td><td>JW Pharmaceutical</td><td></td><td>Normal saline</td></tr><tr><td>Isoflurane</td><td>Hana Med</td><td></td><td>Isoflurane</td></tr></tbody></table>

reproducible motor deficit following aortic occlusion in a rat model of spinal cord ischemia

Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia

Spinal cord ischemia is a fatal complication following thoracoabdominal aortic aneurysm surgery. Researchers can investigate the strategies for preventing and treating this complication using experimental models of spinal cord ischemia. The model described here demonstrates varying degrees of paraplegia that relate to the length of occlusion following thoracic aortic occlusion in a rat spinal cord ischemia model.
A 2-Fr. balloon-tipped catheter was advanced through the femoral artery into the descending thoracic aorta until the catheter tip was placed at the left subclavian artery in anesthetized male Sprague-Dawley rats. Spinal cord ischemia was induced by inflating the catheter balloon. After a set period of occlusion (9, 10, or 11 min), the balloon was deflated. Neurologic assessment was performed using the motor deficit index at 24 h after surgery, and the spinal cord was harvested for histopathological examination.
Rats that underwent 9 min of aortic occlusion showed mild and reversible motor impairment in the hind limb. Rats subjected to 10 min of aortic occlusion presented with moderate but reversible motor impairment. Rats subjected to 11 min of aortic occlusion displayed complete and persistent paralysis. The motor neurons in the spinal cord sections were more preserved in rats subjected to shorter duration of aortic occlusion.
Researchers can achieve a reproducible hind limb motor deficit following thoracic aortic occlusion using this spinal cord ischemia model.

During a period of spinal cord ischemia, aortic occlusion was performed for 9 min (n=3), 10 min (n=3), or 11 min (n=3). The motor deficit index in rats is presented in Table 2. Rats that underwent 9 min of aortic occlusion showed a mild and reversible motor impairment in the hind-limb. Rats subjected to 10 min of aortic occlusion presented with moderate motor deficit, but not complete paralysis. Rats that underwent 11 min of occlusion time displayed complete and persistent paralysis.
Represent...

This study demonstrates the technique to make a minimally invasive and easily reproducible model of spinal cord ischemia in rats. Various degrees of hind limb motor deficit can be produced by controlling the aortic occlusion time.

Spinal cord ischemia is a fatal complication following thoracoabdominal aortic aneurysm surgery. Researchers can investigate the strategies for preventing ...

The overall goal of this procedure is to present the technique to make a spinal cord ischemia model in a rat. Demonstrating the procedure will be done by Mi-Sun Lim. The catheter placed in tail artery is used for monitoring of arterial pressure.After completing the catheterization, advance the Fogarty catheter through the femoral artery into the descending thoracic aorta. To induce spinal cord ischemia, inflate the Fogarty catheter and drain the blood flow from the carotid artery into the external blood reservoir for controlling proximal arterial pressure. After the scheduled occlusion time, deflate the Fogarty catheter balloon, reinfuse the drained blood and remove the femur catheter.Once the rat is anesthetized, place the rat in the operating table in the ventral position and maintain anesthesia with spontaneous respiration using a facial mask of inhaled isoflurane Make a horizontal skin incision on the right inguinal area. Using a 4.0 black silk suture, place a loose tie on both proximal and distal ends of the artery to maximize the exposure. While monitoring the arterial pressure through the tail artery, remove the catheters from the femoral and carotid arteries and close the wound with silk sutures.Hind-limb motor function can be assessed using the motor deficit index. The motor deficit index is defined as the sum of the scores in relation with the lower extremities plus placing/stepping reflex and the maximum deficit is indicated by a score of six. At the assessment of hind-limb motor function at 24 hour after surgery, rats that underwent nine minutes of aortic occlusion showed mild and reversible motor impairment in the hind-limb.Rats subjected to 10 minutes of aortic occlusion presented moderate motor deficit, but not complete paralysis. Rats that underwent 11 minutes of occlusion time, displayed complete and persistent paralysis. You can induce various degrees of hind-limb motor deficit by controlling the aortic occlusion time using this spinal cord ischemia model.Once mastered, this technique can be performed within 40 minutes. Thank you for watching and good luck with your experiment.

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Research

JoVE Journal

Medicine

Surgical induction of ischemic brain damage in the rat is a widely used model for stroke research. Here we demonstrate the induction of focal cerebral ischemia by occlusion of the middle cerebral artery. Visualization of the resulting infarct by histological staining and magnetic resonance imaging is also shown.

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

Bilateral carotid occlusion coupled with systemic hypotension produces global brain ischemia in the rat, resulting in damage to the hippocampus with reproducible severity. Animal subjects are impaired with predictable patterns of brain damage, they recover expediently, and mortality rates are comparatively low.

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

The lack of mechanistic understanding of spinal cord ischemia-reperfusion injury has hindered further adjuncts to prevent paraplegia following high risk aortic operations. Thus, the development of animal models is imperative. This manuscript demonstrates reproducible lower extremity paralysis following thoracic aortic occlusion in a murine model.

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

Here we present a protocol to produce permanent distal middle cerebral artery occlusion in elderly female rats with simultaneous occlusion of the carotid arteries to generate large cortical infarcts and sustained deficits. We show confirmation of the lesion size using structural MRI at 24 hr and 8 weeks after stroke.

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

A mouse model of cerebral ischemia-reperfusion is established to investigate the pathophysiology of stroke. We distally ligate the right middle cerebral artery and right common carotid artery and restore blood flow after 10 or 40 min of ischemia.

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

The purpose of this study is to establish and validate an animal model for research in the recovery and sequela stages of brain ischemia by testing brain infarction and sensorimotor function after middle cerebral artery occlusion/reperfusion (MCAO/R) after 1-90 days in rats.

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

Spinal cord microcirculation plays a pivotal role in spinal cord injury. Most methods do not allow real-time assessment of spinal cord microcirculation, which is essential for the development of microcirculation-targeted therapies. Here, we propose a protocol using Laser-Doppler-Flow Needle probes in a large animal model of ischemia/reperfusion.

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion

The current protocol describes a rat model of middle cerebral artery occlusion/reperfusion that preserves the anatomical structure of cerebral vessels without causing damage.

A Rat Model of Middle Cerebral Artery Occlusion/Reperfusion Without Damaging the Anatomical Structure of Cerebral Vessels

This protocol describes the process of preparing for middle cerebral artery occlusion reperfusion via the common carotid artery.

A Modified Model Preparation for Middle Cerebral Artery Occlusion Reperfusion

The objective of this study was to establish a reproducible method for performing a craniectomy followed by permanent, selective middle cerebral artery occlusion in rats for application in experimental studies of motor and spatial memory deficits.

Selective and Permanent Occlusion of the Middle Cerebral Artery in Rats: An Experimental Approach for Studying Motor and Spatial Memory Deficits

Learn a minimally invasive technique to create a reproducible spinal cord ischemia model in rats using Fogarty catheter occlusion. This method enables controlled study of hind limb motor deficits for neuroscience research.

Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia

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