JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
从天然产物其生物合成基因簇:一个演示使用Polyketomycin从<em>链diastatochromogenes</em>Tü6028
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from
Streptomyces diastatochromogenes
Tü6028
Please note that all translations are automatically generated.
Click here for the English version.
从天然产物其生物合成基因簇:一个演示使用Polyketomycin从<em>链diastatochromogenes</em>Tü6028
DOI:
10.3791/54952-v
•
09:08 min
•
January 13, 2017
•
Anja Greule
,
Songya Zhang
,
Thomas Paululat
,
Andreas Bechthold
1
Department of Pharmaceutical Biology and Biotechnology
,
Albert-Ludwigs-Universität Freiburg, Germany
,
2
Department of Chemistry and Biology
,
Universität Siegen
Chapters
00:05
Title
00:54
Identification of a Natural Product with Antibiotic Property
03:54
Propose Biosynthetic Model of the New Isolated Compound
05:02
Identification of the Biosynthetic Gene Cluster
06:34
Verification of the Biosynthetic Gene Cluster
07:40
Results: HPLC Analysis of
S. diastatochromogenes
with Inactivated
pokPI
Gene
08:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这里我们介绍(一)鉴定抗菌活性的天然产物的,(B)化合物的净化,(C),其生物合成的第一种模式,(D)的基因组测序/ -mining和详细协议( E)的生物合成基因簇的验证。
Tags
Natural Product
Biosynthetic Gene Cluster
Polyketomycin
Streptomyces Diastatochromogenes
Antibiotic Activity
Genome Mining
Streptomyces
Antibiotic Producer
Biosynthetic Pathway
Compound Extraction
HPLC
Disc Diffusion Assay
Test Strain
Antibiotic
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
用GFP标记TMEM184A构建肝素受体身份确认
斑马鱼 RNASeq-based 基因表达数据的样品制备与分析
CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究
无基因克隆的基因破坏的
变形链球菌
菌株的生成
从土壤、根际和根 Endosphere 提取细菌群落数据的根菌群探析
间歇酵母 2-混合筛序列数据的信息化分析
利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
基于荧光的感染组织细菌基因调控方法
remtoe 控制系统在内源基因表达调控中的应用
基于质谱的蛋白质组学分析, 利用 OpenProt 数据库揭开从非规范开放阅读框架翻译的新蛋白质
Read Article