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从天然产物其生物合成基因簇:一个演示使用Polyketomycin从<em>链diastatochromogenes</em>Tü6028
JoVE Journal
Genetics
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JoVE Journal Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
DOI:

09:08 min

January 13, 2017

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Chapters

  • 00:05Title
  • 00:54Identification of a Natural Product with Antibiotic Property
  • 03:54Propose Biosynthetic Model of the New Isolated Compound
  • 05:02Identification of the Biosynthetic Gene Cluster
  • 06:34Verification of the Biosynthetic Gene Cluster
  • 07:40Results: HPLC Analysis of S. diastatochromogenes with Inactivated pokPI Gene
  • 08:07Conclusion

Summary

Automatic Translation

这里我们介绍(一)鉴定抗菌活性的天然产物的,(B)化合物的净化,(C),其生物合成的第一种模式,(D)的基因组测序/ -mining和详细协议( E)的生物合成基因簇的验证。

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