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Grootbrengen en Double-stranded RNA-gemedieerde gen knock-down in het verbergen Kever,

Article DOI: 10.3791/54976-v • 09:57 min • December 28th, 2016
December 28th, 2016 •
1,2, 1, 1,2
1Entomology Department, University of Maryland, 2Program in Molecular and Cell Biology, University of Maryland

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Hier presenteren we protocollen voor het kweken van een intermediair-kiem kever, Dermestes maculatus (D. maculatus) in het lab. We hebben ook protocollen voor de embryonale en ouderlijke RNAi en methodes te delen voor het analyseren van embryonale fenotypes voor bestudering van genfunctie in deze soort.

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Genetics RNAi gen knock-down insect kever, Segmentatie pair-rule-gen embryo micro-injectie dsRNA evo-devo
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