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Developmental Biology

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和无血清收集无饲养小鼠胚胎干细胞条件培养基的无细胞方法
 

和无血清收集无饲养小鼠胚胎干细胞条件培养基的无细胞方法

Article doi: 10.3791/55035
January 8th, 2017 Usage Statistics

Summary January 8th, 2017

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这个协议提供了小鼠胚胎干细胞的血清衍生的集合(MESC)-conditioned培养基(MESC-CM)的方法(胎牛血清,FBS) - 和馈线(小鼠胚胎成纤维细胞,MEF中)用于小区-free条件 - 免费的做法。它可以是适用于老化和老化有关的疾病的治疗。

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