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Developmental Biology
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定义的无异和无饲养培养条件的人 iPSC 视网膜细胞模型的生成
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JoVE Journal
Developmental Biology
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
Please note that all translations are automatically generated.
Click here for the English version.
定义的无异和无饲养培养条件的人 iPSC 视网膜细胞模型的生成
DOI:
10.3791/57795-v
•
11:29 min
•
September 06, 2018
•
Amélie Slembrouck-Brec*
1
,
Céline Nanteau*
1
,
José-Alain Sahel
,
Olivier Goureau
,
Sacha Reichman
1
Institut de la Vision
,
Sorbonne Université, INSERM, CNRS, F-75012 Paris, France
Chapters
00:04
Title
01:04
Generation and Maturation of Retinal Organoids
02:50
Generation and Amplification of Human iPSC-derived RPE (hiRPE) Cells
05:17
Cryopreservation of Retinal Organoids and hiRPE Cells
07:09
Thawing of Retinal Organoids and hiRPE Cells
09:01
Results: Generation of All Neural Retina Cell Types and RPE
10:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多潜能干细胞的专业视网膜细胞的产生是干细胞治疗视网膜疾病的一个转折点。本文介绍了一种有效生成视网膜 organoids 和视网膜色素上皮的简单方法, 用于基础、平移和临床研究。
Tags
IPS Cells
Retinal Cell Models
Retinal Organoids
Retinal Pigmented Epithelial Cells
RPE Cells
Xeno-free
Feeder-free
Differentiation
Culture Conditions
Cell Isolation
Floating Culture
Pro B-27 Medium
Pro N-2 Medium
FGF2
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