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Developmental Biology
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JoVE Journal Developmental Biology
Evaluering av intracellulær plasseringen av reaktive oksygen arter i Solea Senegalensis Spermatozoa
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Evaluering av intracellulær plasseringen av reaktive oksygen arter i Solea Senegalensis Spermatozoa

Article DOI: 10.3791/55323-v • 10:24 min • March 11th, 2018
March 11th, 2018 •
1,2, 2,3
1Department of Molecular Biology, University of León, 2INDEGSAL, University of León, 3Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

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Denne protokollen beskriver en detaljert metodikk for å oppdage H2O2 lokalisering i Solea senegalensis spermatozoa bruker en følsom fluorochrome DCFH-DA for ROS, en live mitokondrier flekk for mitokondrier og DAPI for kjerner visualisering, henholdsvis. Protokollen er utformet som skal utføres innen 2 timer med enten friske eller tinte spermatozoa.

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Utviklingspsykologi biologi problemet 133 Solea Senegalensis Spermatozoa mitokondrier kryonisk bevaring mitokondrier flekk DCFH-DA DAPI AC Confocal mikroskopi
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