JoVE Journal
Genetics
Genetics
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Summary
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PCR介导的基因修饰可用于产生在念珠菌属种类,这有利于可视化和酵母细胞和蛋白质的定量荧光蛋白融合体。在此,我们提出了在近平滑念珠菌构建荧光蛋白融合(左心室Eno1-FP)的战略。