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Genetics

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Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
 

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

Article DOI: 10.3791/63177-v 10:08 min December 21st, 2021
December 21st, 2021

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Summary

Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.

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Maternally-expressed Genes Early Development Maternal CRISPR Technique Maternal Effect Genes Multiplexing Guide RNAs Maternal Effect Phenotypes MRNA Transcripts Gametes Embryogenesis Micro Injection Somatic Mutations Guide RNA Template Oligonucleotide T4 DNA Polymerase DNA Cleanup And Concentrator Kit SgRNA Mixture In-vitro T7 Transcription Kit Agarose Gel Ethidium Bromide Tris-Borate-EDTA Gel
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