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Journal / Neuroscience / Rekombinant α-β ve γ Synucleins Protein fosfataz…
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Neuroscience
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JoVE Journal Neuroscience
Rekombinant α-β ve γ Synucleins Protein fosfataz 2A katalitik alt birim faaliyet hücre ücretsiz deneyleri teşvik
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Rekombinant α-β ve γ Synucleins Protein fosfataz 2A katalitik alt birim faaliyet hücre ücretsiz deneyleri teşvik

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

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Bu yayın rekombinant α-synuclein, β-synuclein veya basit bir kolorimetrik yöntemi kullanarak γ-synuclein protein karşılık olarak rekombinant protein fosfataz 2A katalitik alt birimi (PP2Ac) etkinliğini ölçmek nasıl gösteren bir protokolü sunar. Fare beyninde PP2Ac doğru α-synuclein özgüllük ile ilgili bulgular göstermektedir.

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Neuroscience sayı: 126 Renkölçer tahlil dephosphorylation malakit fosfat fosfataz aktivitesi PP2A katalitik alt birimi rekombinant proteinler synucleins treonin phosphopeptide
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