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Journal / Neuroscience / Rekombinant α-β - och γ-Synucleins stimulera…
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JoVE Journal Neuroscience
Rekombinant α-β - och γ-Synucleins stimulera Protein 2A katalytisk subenhet fosfatasaktiviteten Cell gratis analyser
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Rekombinant α-β - och γ-Synucleins stimulera Protein 2A katalytisk subenhet fosfatasaktiviteten Cell gratis analyser

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

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Denna skrift presenterar ett protokoll som visar hur man mäter aktiviteten av rekombinant protein fosfatas 2A katalytisk subenhet (PP2Ac) som svar på rekombinant α-synuclein, β-synuclein eller γ-synuclein proteiner med en enkel kolorimetriska analys. Vi visar slutsatser angående α-synuclein specificitet mot PP2Ac i mus hjärnan.

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Neurovetenskap fråga 126 kolorimetriska analys Defosforylering malakit fosfat fosfatasaktiviteten PP2A katalytisk subenhet rekombinanta proteiner synucleins treonin phosphopeptide
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