<em>In Vitro</em> Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

Nohemi Salinas-Jazm&#237;n; Edith Gonz&#225;lez-Gonz&#225;lez; Luz X. V&#225;squez-Bochm; Sonia M. P&#233;rez-Tapia; Marco A. Velasco-Vel&#225;zquez

doi:10.3791/55542

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In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

<em>In Vitro</em> Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

17,669 Views

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07:25 min

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May 04, 2017

DOI:

10.3791/55542-v

DOI:

10.3791/55542-v

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07:25 min

May 04, 2017

•

1 Views

Nohemi Salinas-Jazmín^1,2, Edith González-González¹, Luz X. Vásquez-Bochm³, Sonia M. Pérez-Tapia^4,5, Marco A. Velasco-Velázquez⁶

¹Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences,National Polytechnic Institute (IPN), University of Mexico (UNAM), ²School of Chemistry,National Autonomous University of Mexico (UNAM), ³Graduate Program in Chemical Sciences,National Autonomous University of Mexico (UNAM), ⁴Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences,National Polytechnic Institute (IPN), ⁵Department of Immunology, National Scool of Biological Sciences,National Polytechnic Institute (IPN), ⁶Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine,National Autonomous University of Mexico (UNAM)

Transcript

The overall goal of this procedure is to compare two key functional characteristics between therapeutic antibodies, target binding, and CDC induction during their biosimilar development, or as a quality control, during their production.These methods can help answer our key questions about how to assess functional differences between therapeutic antibodies that bind the same target protein.These techniques are quick, simple, and inexpensive, allowing for day execution in most laboratories.Demonstrating the procedures will be Edith Gonz

Summary

This protocol describes the in vitro comparison of two key functional characteristics of rituximab: target binding and complement-dependent cytotoxicity (CDC) induction. The methods were employed for a side-to-side comparison between reference rituximab and a rituximab biosimilar. These assays can be employed during biosimilar development or as a quality control in their production.