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Journal / Biochemistry / Spannklemme Fluorometrie in
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JoVE Journal Biochemistry
Spannklemme Fluorometrie in
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Spannklemme Fluorometrie in

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

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Dieser Artikel beschreibt eine Verbesserung der konventionellen Voltage-Clamp Fluorometry (VCF), bei der fluoreszierende unnatürliche Aminosäuren (fUAA) anstelle von Maleinimidfarbstoffen verwendet werden, um strukturelle Umlagerungen in Ionenkanälen zu untersuchen. Das Verfahren umfasst Xenopus- Oozyten-DNA-Injektion, RNA / fUAA-Coinjektion und gleichzeitige Strom- und Fluoreszenzmessungen.

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Biochemie Ausgabe 123 Fluoreszierende unnatürliche Aminosäuren Spannungskammer-Fluorometrie aufgeschnittene Oozytenspannungsklemme Anap Bernstein-Stop-Codon-Suppression DNA-Mikroinjektion,
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