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Journal / Biochemistry / Spenningsklemme Fluorometri i
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Biochemistry

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JoVE Journal Biochemistry
Spenningsklemme Fluorometri i
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Spenningsklemme Fluorometri i

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

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Denne artikkelen beskriver en forbedring av konvensjonell spenningsklemme-fluorometri (VCF) der fluorescerende unaturlige aminosyrer (fUAA) brukes i stedet for maleimidfarger, for å undersøke strukturelle omarrangementer i ionkanaler. Prosedyren inkluderer Xenopus oocyt DNA-injeksjon, RNA / fUAA myntutjaging, og samtidige strøm- og fluorescensmålinger.

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Biokjemi utgave 123 fluorescerende unaturlige aminosyrer spenningsklemmefluorometri kutt-åpen oocytspenningsklemme anap amberstoppkodonundertrykkelse DNA-mikroinjeksjon,
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