Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / LERLIC-MS / MS Gedetailleerde karakterisering en…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
LERLIC-MS / MS Gedetailleerde karakterisering en kwantificering van glutamine en asparagine Deamidatie in Shotgun Proteomics
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Journal (Biochemistry)
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
Journal (Biochemistry)
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
 
Click here for the English version

LERLIC-MS / MS Gedetailleerde karakterisering en kwantificering van glutamine en asparagine Deamidatie in Shotgun Proteomics

Article DOI: 10.3791/55626-v • 08:01 min • April 9th, 2017
April 9th, 2017 •
*1, *1, 1
1Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier geven we een stap-voor-stap protocol van de lange lengte elektrostatische repulsie-hilic-tandem massaspectrometrie (LERLIC-MS / MS) methode. Dit is een nieuwe methode die het mogelijk maakt voor het eerst kwantificering en karakterisering van glutamine en asparagine deamidering isovormen door shotgun proteomics.

Tags

Biochemistry Deamidatie glutamine asparagine Erlić LERLIC degeneratieve eiwitmodificaties DPM posttranslationele modificaties PTMs transamideren lange lengte anionenuitwisselende LAX Shotgun proteomics HILIC.
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter