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Journal / Biochemistry / ショットガンプロテオミクスにある深さの特徴付けのためLERLIC-MS /…
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Biochemistry

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JoVE Journal Biochemistry
ショットガンプロテオミクスにある深さの特徴付けのためLERLIC-MS / MSおよびグルタミンの定量化およびアスパラギン脱アミド
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Journal (Biochemistry)
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Identification of Functional Protein Regions Through Chimeric Protein Construction
Journal (Biochemistry)
Identification of Functional Protein Regions Through Chimeric Protein Construction
 
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ショットガンプロテオミクスにある深さの特徴付けのためLERLIC-MS / MSおよびグルタミンの定量化およびアスパラギン脱アミド

Article DOI: 10.3791/55626 • 08:01 min
April 9th, 2017 •
*1, *1, 1
1Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University
* These authors contributed equally

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April 9th, 2017

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ここでは、長尺の静電反発力 - 親水性相互作用クロマトグラフィー - タンデム質量分析（LERLIC-MS / MS）方法のステップバイステップのプロトコルを提示します。これは、ショットガンプロテオミクスによってグルタミンとアスパラギン脱アミド化アイソフォームを初めて定量および特徴付けのために有効に小説の方法論です。

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