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Journal / Neuroscience / Elektrofysiologisk metode til hele celle spænding …
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Elektrofysiologisk metode til hele celle spænding clamp optagelser fra
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Elektrofysiologisk metode til hele celle spænding clamp optagelser fra

Article DOI: 10.3791/55627-v • 10:36 min • June 13th, 2017
June 13th, 2017 •
*1, *1, 1, 2, 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, 2Department of Physiology, Development and Neuroscience, University of Cambridge
* These authors contributed equally

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Helecelleoptagelser fra Drosophila melanogaster fotoreceptorer muliggør måling af spontane mørke humper, kvantebumps, makroskopiske responser til lys og aktuelle spændingsforhold under forskellige forhold. I kombination med D. melanogaster genetiske manipulationsværktøjer muliggør denne metode undersøgelsen af ​​den allestedsnærværende inositol-lipid-signalvej og dens mål, TRP-kanalen.

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Neurovidenskab udgave 124 helcelleoptagelser spændingsklemme strømklemme elektrofysiologi, Fototransduktion enkeltcelle Ca intracellulær perfusion
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