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Journal / Biochemistry / Isolering af Intermediate Filament Proteiner fra…
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Biochemistry

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JoVE Journal Biochemistry
Isolering af Intermediate Filament Proteiner fra Multiple Musvæv til Studie Aging-associerede Post-translationelle Modifikationer
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Isolering af Intermediate Filament Proteiner fra Multiple Musvæv til Studie Aging-associerede Post-translationelle Modifikationer

Article DOI: 10.3791/55655-v • 09:29 min • May 18th, 2017
May 18th, 2017 •
1, 1, 1, 1, 1
1Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill

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I denne metode præsenterer vi biokemiske procedurer til hurtig og effektiv isolering af intermediære filament (IF) proteiner fra flere musevæv. Isolerede IF'er kan anvendes til at studere ændringer i posttranslationelle modifikationer ved massespektrometri og andre biokemiske analyser.

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Biochemistry intermediary filamenter post-translationel modifikation massespektrometri immunpræcipitation aldring stress dyremodeller vævsfraktionering
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