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从多个小鼠组织分离中间丝状蛋白以研究与老化相关的翻译后修饰
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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从多个小鼠组织分离中间丝状蛋白以研究与老化相关的翻译后修饰

DOI:
10.3791/55655-v

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09:29 min

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May 18, 2017

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, , , ,
1Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:49Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
  • 03:16Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
  • 07:15Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

在这种方法中，我们提出了从多个小鼠组织快速高效地分离中间丝（IF）蛋白质的生物化学方法。分离的IF可用于通过质谱法和其他生化测定法研究翻译后修饰的变化。

Tags

Intermediate Filament ProteinsPost-translational ModificationsTriton X-100 BufferHigh Salt BufferPBS EDTA BufferSDS Sample BufferCentrifugationHomogenization

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