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Fraccionamiento de Membranas Cerebrales: Un<em> Ex Vivo</em> Enfoque para evaluar la localización de proteínas subsinápticas
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
DOI:

09:49 min

May 12, 2017

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Chapters

  • 00:00Title
  • 00:18Introduction
  • 02:22Obtaining Mouse Brain Synaptosomes Using a Discontinuous Sucrose Gradient
  • 04:37Pre-post and Extra-synaptic Fraction Isolation
  • 06:52Analyze Samples and Validate Fractionation
  • 07:26Representative Results
  • 08:53Conclusion

Summary

Automatic Translation

Aquí, presentamos un protocolo de fraccionamiento de membrana cerebral que representa un procedimiento robusto para aislar proteínas pertenecientes a diferentes compartimentos sinápticos.

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