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Culturas primárias mistas de Murino Intestino Delgado destinadas ao estudo de Gut hormonal Secreção e imagens de células vivas de Células Enteroendócrinas

Article DOI: 10.3791/55687-v • 09:16 min • April 20th, 2017
April 20th, 2017 •
*1, *1, 1, 1, 1
1Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
* These authors contributed equally

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Este protocolo descreve o isolamento e cultura de células do intestino delgado de murídeo mistos. Estas culturas primárias intestinais permitir a investigação das vias de sinalização subjacentes a secreção do péptido intestino utilizando um número de técnicas.

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Cellular Biology Edição 122 Intestino delgado duodeno rato primário cultura enteroendrina células L intestino hormona
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