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小鼠小肠的混合原代培养拟肠激素分泌的研究和活细胞成像的肠内分泌细胞的供

JoVE Journal
Biology

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JoVE Journal Biology

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Please note that all translations are automatically generated. Click here for the English version.

小鼠小肠的混合原代培养拟肠激素分泌的研究和活细胞成像的肠内分泌细胞的供

DOI:

10.3791/55687-v

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09:16 min

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April 20, 2017

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Arianna Psichas*¹, Gwen Tolhurst*¹, Cheryl A. Brighton, Fiona M. Gribble, Frank Reimann

¹Metabolic Research Laboratories and MRC Metabolic Diseases Unit,Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

Chapters

00:05Title
00:54Tissue Preparation
03:15Removal of Cell Debris and Single Cells
04:44Collection of Crypt Fragments
06:55Intestinal Culture Plating
07:38Results: Secretion and Imaging Using Primary Small Intestinal Cultures
08:36Conclusion

Summary

Automatic Translation

这个协议描述混合鼠小肠细胞的分离和培养。这些一次肠培养使底层使用多种技术肠肽分泌信号转导途径的研究。

Tags

Keywords: Enteroendocrine Cells Gut Hormone Secretion Live Cell Imaging Mixed Primary Cultures Murine Small Intestine Intestinal Epithelium Intestinal Contents Muscle Layer Epithelium Chyme Mucus

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