Journal
/
Neuroscience
/
The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

DOI:
10.3791/55737-v

•

07:55 min

•

July 09, 2017

•

, ,
1Research Laboratory for Stereology and Neuroscience,Bispebjerg-Frederiksberg Hospital, 2Institute of Clinical Medicine, Department of Health and Medical Sciences,University of Copenhagen

Chapters

  • 00:05Title
  • 00:52Brain Tissue Processing
  • 02:11Immunostaining
  • 04:49Estimation of Total Number of BrdU-labeled Neurons Using the Optical Fractionator
  • 06:46Results: Total Number of BrdU-positive Neurons in the GCL-SGZ of the Rat Hippocampus
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a stereological method, the optical fractionator, used to quantify the formation of new neurons, and their survival, in the rat hippocampus following electroconvulsive stimulation. When correctly implemented, the sensitivity and efficiency of stereological methods ensures accurate estimates with a fixed and predetermined precision.

Tags

Keywords: Optical FractionatorStereological StudyCell Number EstimationRat ModelElectroconvulsive TherapyQuantitative AnalysisBrain AreaUnbiased SamplingFixed PrecisionBrain HemispheresHippocampusImmunostainingCryostat SectioningCryoprotectant

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55737?article_type=v

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique
check_url/55737?article_type=v

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination
check_url/55737?article_type=v

Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
check_url/55737?article_type=v

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
check_url/55737?article_type=v

The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders
check_url/55737?article_type=v

Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment
check_url/55737?article_type=v

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model – Introduction of the Surgical Technique
check_url/55737?article_type=v

Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration
check_url/55737?article_type=v

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model
check_url/55737?article_type=v

Applying the RatWalker System for Gait Analysis in a Genetic Rat Model of Parkinson’s Disease

Read Article