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Protokoller til C-Brick DNA Standard Assembly ved anvendelse af Cpf1
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Protokoller til C-Brick DNA Standard Assembly ved anvendelse af Cpf1

Article DOI: 10.3791/55775-v • 12:03 min • June 15th, 2017
June 15th, 2017 •
1, 1, 1
1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

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CRISPR-associeret protein Cpf1 kan styres af et specielt designet CRISPR-RNA (crRNA) til spaltning af dobbeltstrenget DNA på ønskede steder, der genererer klæbrige ender. Baseret på denne egenskab blev en DNA-samlingsstandard (C-Brick) etableret, og en protokol, der beskriver dens anvendelse, er beskrevet her.

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Genetik udgave 124 syntetisk biologi CRISPR Cpf1 C-Brick klæbrige ender DNA-samling, Monteringsstandard
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