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Journal
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Immunology and Infection
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来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
Please note that all translations are automatically generated.
Click here for the English version.
来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
DOI:
10.3791/55857-v
•
07:35 min
•
June 07, 2017
•
James Weger-Lucarelli
,
Nisha K. Duggal
,
Aaron C. Brault
,
Brian J. Geiss
,
Gregory D. Ebel
1
Department of Microbiology, Immunology, and Pathology
,
Colorado State University
,
2
Division of Vector-Borne Diseases
,
Centers for Disease Control and Prevention
Chapters
00:05
Title
00:40
Preparation of
In Vitro
Transcribed RNA
02:54
Rescue of Infectious Virus by Electroporation
05:56
Results:
In Vitro
and
In Vivo
Characteristics of Clone-derived Virus
07:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案描述了从双质粒感染性cDNA克隆中恢复感染性Zika病毒。
Tags
Zika Virus
Infectious Clone
Recombinant Virus
Flavivirus
Plasmid Digestion
Ligation
In Vitro Transcription
Vero Cells
Electroporation
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