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Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
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Neuroscience
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JoVE Journal Neuroscience
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

DOI:
10.3791/57543-v

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09:32 min

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May 21, 2018

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, ,
1Program in Molecular and Cellular Biology,State University of New York Downstate Medical Center, 2Department of Neurology and Rehabilitation,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 00:40Mouse Brain Cortex Dissociation
  • 04:49O4+ Oligodendrocyte Isolation and Plating
  • 07:26Results: Representative Cell Surface Marker Expression During Mouse Oligodendrocyte Development
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the immunomagnetic isolation of primary mouse oligodendrocytes, which allows the rapid and specific isolation of the cells for in vitro culture.

Tags

Keywords: Immunomagnetic IsolationMouse Primary OligodendrocytesMyelinMyelinationNeural ScienceCell CultureDissociationNeurobasal A MediumBovine Growth SerumDNase IPipetting

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