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Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis
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JoVE Journal Neuroscience
Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis

Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/56032-v

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13:02 min

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November 04, 2017

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, , , ,
1Pharmacology,Dalhousie University, 2Medical Neuroscience,Dalhousie University, 3Psychiatry,Dalhousie University

Chapters

  • 00:00Title
  • 01:14Constructing the Markers
  • 03:12Prepare the Animal for Recording
  • 05:51Gait Recording
  • 07:10EAE Mouse Example 1
  • 07:28EAE Mouse Example 2
  • 07:48Examples of poor walking
  • 08:48Analysis
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

Kinematic gait analysis in the sagittal plane yields highly precise information about how movement is executed. We describe the application of these techniques to identify gait deficits for mice subjected to autoimmune-mediated demyelination. These methods may also be used to characterize gait deficits for other mouse models featuring impaired locomotion.

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Keywords: Kinematic Gait AnalysisC57BL/6 MiceMOG35-55Experimental Autoimmune Encephalomyelitis (EAE)Multiple SclerosisNeurological DeficitsMotor StabilityClinical ScoringWhite Matter LossReflective MarkersHigh-speed CameraMotion Analysis SoftwareAnatomical PointsHind LegAdhesive SurfaceHot Glue Gun

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