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Biochemistry

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JoVE Journal Biochemistry
Et modifisert gjær-ett hybrid system for Heteromer Proteinkompleks-DNA-interaksjonsstudier
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Et modifisert gjær-ett hybrid system for Heteromer Proteinkompleks-DNA-interaksjonsstudier

Article DOI: 10.3791/56080-v • 10:47 min • July 24th, 2017
July 24th, 2017 •
1, 2, 1
1Department of Neurology, University of Southern California, 2Section of Cell and Developmental Biology, University of California San Diego

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Den modifiserte gjær-en-hybrid-analysen beskrevet her er en forlengelse av den klassiske gjær-en-hybrid-analysen (Y1H) for å studere og validere den heteromere proteinkompleks-DNA-interaksjonen i et heterologt system for enhver funksjonell genomisk studie.

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Biochemistry Protein-DNA-interaksjon gjærgenetikk gjær-en-hybrid heterologt system β-galaktosidase reporter transkripsjonsfaktorer transkripsjonsregulering.
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