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O murino colina deficiente, Ethionine-completada (CDE) dieta modelo de lesão hepática crônica
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O murino colina deficiente, Ethionine-completada (CDE) dieta modelo de lesão hepática crônica

Article DOI: 10.3791/56138-v • 07:28 min • October 21st, 2017
October 21st, 2017 •
1, 1, 2, 3, 1,4, 1, 5,6,7, 8,9, 10,11, 1,12
1School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute, Curtin University, 3Institute of Biochemistry, Christian-Albrechts-University, 4School of Veterinary and Life Sciences, Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences, The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences, Edith Cowan University, 12School of Medicine and Pharmacology, University of Western Australia

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Aqui nós descrevemos um método comum para induzir lesão hepática crônica em ratos, alimentando-se de uma dieta deficiente em colina e ethionine-complementado do (CDE). Demonstramos que o monitoramento de integridade, perfusão hepática, isolamento e preservação. Um curso de tempo de seis semanas pode informar sobre a lesão hepática, pathohistology, fibrose, inflamatória e respostas de células progenitoras de fígado.

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Biologia celular edição 128 modelo murino dieta deficiente em colina ethionine-completada hepatopatia crônica perfusão hepática fígado isolamento células progenitoras fígado resposta inflamatória histologia fibrose fígado microambiente
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