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Análise de espectrometria de massa para identificar a ubiquização do CENP-A marcado pelo EYFP (EYFP-CENP-A)
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Análise de espectrometria de massa para identificar a ubiquização do CENP-A marcado pelo EYFP (EYFP-CENP-A)

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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A ubiquização CENP-A é um importante requisito para a deposição do CENP-A no centromere, herdada por dimerização entre divisão celular e indispensável à viabilidade celular. Aqui descrevemos a análise da espectrometria em massa para identificar a ubiquização da proteína CENP-A (EYFP-CENP-A) marcada pela EYFP.

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Biologia Edição 160 centromere kinetochore mitose CENP-A modificação pós-translacional PTM ubiquização análise de espectrometria de massa
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