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3D 细胞培养和非抗凝全血对内皮细胞抗凝及抗炎作用的评价
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Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
Please note that all translations are automatically generated.
Click here for the English version.
3D 细胞培养和非抗凝全血对内皮细胞抗凝及抗炎作用的评价
DOI:
10.3791/56227-v
•
07:08 min
•
September 05, 2017
•
Riccardo Sfriso
2
,
Anjan Bongoni
,
Yara Banz
,
Nikolai Klymiuk
,
Eckhard Wolf
,
Robert Rieben
1
Department of Clinical Research
,
University of Bern
,
2
Graduate School for Cellular and Biomedical Sciences
,
University of Bern
,
3
Immunology Research Centre
,
St. Vincent’s Hospital Melbourne
,
4
Institute of Pathology
,
University of Bern
,
5
Institute of Molecular Animal Breeding and Biotechnology
,
Ludwig-Maximilian University
Chapters
00:05
Title
00:48
Coating Microcarrier Beads
01:39
Collecting Cells
02:26
Seeding Cells into the Stirrer Flask
03:45
Experimental Procedure
04:32
Incubation with Non-anticoagulated Blood
05:46
Results: Clotting
06:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个
体外
模型, 允许对整个 non-anticoagulated 血液中的凝血进行研究和分析。该系统的抗凝血依赖于健康的内皮细胞的天然抗凝作用, 内皮细胞活化会导致血栓形成。
Tags
3D Cell Culture
Endothelial Cells
Anticoagulant Properties
Anti-inflammatory Properties
Whole Blood
Non-anticoagulated Blood
Cardiovascular Research
Transportation Research
Surgery Research
Anti-immunity
Endothelial Cell Activation
Microcarrier Beads
Coagulation
Phosphate Buffered Saline (PBS)
Dulbecco’s Modified Eagle’s Medium (DMEM)
Medium 199
Trypsin
EDTA
Cell Culture Media
Magnetic Stirrer Flask
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