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Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation
JoVE Journal
Genetics
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JoVE Journal Genetics
Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation

Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation

DOI:
10.3791/56245-v

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10:33 min

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September 04, 2017

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, , ,
1CytoGnomix Inc., 2Department of Pathology and Laboratory Medicine,Western University, 3Department of Biochemistry,Western University

Chapters

  • 00:05Title
  • 01:28Preparing Blood Cell Image Sample Sets for Automated Analysis by Software to Automate Dicentric Chromosome Detection and Estimate Radiation Exposure
  • 03:32Analyzing the Prepared Samples
  • 05:00Dose Estimation and Reports
  • 07:24Results: Testing the Software
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

The cytogenetic dicentric chromosome (DC) assay quantifies exposure to ionizing radiation. The Automated Dicentric Chromosome Identifier and Dose Estimator software accurately and rapidly estimates biological dose from DCs in metaphase cells. It distinguishes monocentric chromosomes and other objects from DCs, and estimates biological radiation dose from the frequency of DCs.

Tags

Keywords: Radiation BiodosimetryDicentric Chromosome IdentificationAutomated Image ProcessingRadiation Dose EstimationMachine LearningCytogenetic AnalysisMetaphase Chromosome ImagesRadiation ExposureCalibration CurveSample ProcessingImage FilteringGenetic Disease Diagnosis

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