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Journal / Genetics / Microinjection di proteine CRISPR/Cas9 in Channel …
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Microinjection di proteine CRISPR/Cas9 in Channel Catfish, Ictalurus punctatus, embrioni per Gene Editing
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Microinjection di proteine CRISPR/Cas9 in Channel Catfish, Ictalurus punctatus, embrioni per Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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Un protocollo di microiniezione semplice ed efficiente per l'editing di gene in embrioni di pesce gatto della manica usando il sistema CRISPR/Cas9 è presentato. In questo protocollo, guida RNA e proteina Cas9 erano microiniettati in tuorlo di embrioni di topo. Questo protocollo è stato convalidato battendo fuori due geni immuno-correlati di pesce gatto della manica.

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Genetica problema 131 CRISPR/Cas9 gene editing microiniezione embrioni di pesce gatto della manica knockout del gene mutazione troncata della proteina indels
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