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Journal / Neuroscience / Live Imaging efterfulgt af enkelt celle inddeling …
JoVE Journal
Neuroscience

This content is Open Access.

JoVE Journal Neuroscience
Live Imaging efterfulgt af enkelt celle inddeling hen til dataskærm cellebiologi og afstamning Progression af flere neurale populationer
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Live Imaging efterfulgt af enkelt celle inddeling hen til dataskærm cellebiologi og afstamning Progression af flere neurale populationer

Article DOI: 10.3791/56291-v • 10:55 min • December 16th, 2017
December 16th, 2017 •
*1,2,3, *1,2,3, *4,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 6, 7, 1,2,3, 1,2,3
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, 6Brain Institute, Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich
* These authors contributed equally

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Et robust protokol til at overvåge neurale populationer af time-lapse video-mikroskopi efterfulgt af software-baserede efterbehandling er beskrevet. Denne metode repræsenterer et kraftfuldt værktøj til at identificere biologiske hændelser i en valgte befolkning under live imaging eksperimenter.

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Neurovidenskab spørgsmålet 130 Live imaging enkelt celle tracking lineage progression voksne neurale stamceller neurale celler slægt træ time-lapse video-mikroskopi
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