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Neuroscience
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活体影像随访单细胞追踪监测细胞生物学及多神经种群的谱系进展
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
Please note that all translations are automatically generated.
Click here for the English version.
活体影像随访单细胞追踪监测细胞生物学及多神经种群的谱系进展
DOI:
10.3791/56291-v
•
10:55 min
•
December 16, 2017
•
Rosa Gómez-Villafuertes*
1,2,3
,
Lucía Paniagua-Herranz*
1,2,3
,
Sergio Gascon*
4,5
,
David de Agustín-Durán
2,3
,
María de la O Ferreras
2,3
,
Juan Carlos Gil-Redondo
2,3
,
María José Queipo
2,3
,
Aida Menendez-Mendez
2,3
,
Ráquel Pérez-Sen
2,3
,
Esmerilda G. Delicado
2,3
,
Javier Gualix
2,3
,
Marcos R. Costa
,
Timm Schroeder
,
María Teresa Miras-Portugal
2,3
,
Felipe Ortega
2,3
1
Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine
,
Complutense University
,
2
University Institute for Neurochemistry Research (IUIN)
,
3
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)
,
4
Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center
,
Ludwig-Maximilians University Munich
,
5
Toxicology and Pharmacology Department, Faculty of Veterinary medicine
,
Complutense University
,
6
Brain Institute
,
Federal University of Rio Grande do Norte
,
7
Department of Biosystems Science and Engineering
,
Eidgenössische Technische Hochschule (ETH) Zurich
Chapters
00:05
Title
01:14
Live Imaging by Time-lapse Video-microscopy
04:02
Post-imaging Immunocytochemistry, Data Collection, and Processing
06:38
Single-cell Tracking
08:56
Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
10:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了一种用延时视频显微镜监测神经种群的鲁棒协议, 并进行了软件后处理。这种方法是一个强有力的工具, 以确定生物事件在选定的人群在现场成像实验。
Tags
Live Imaging
Single Cell Tracking
Neural Populations
Cell Biology
Lineage Progression
Immunocytochemistry
Cell Behavior
Cell Fate
Proliferation
Differentiation
Time-lapse Video-microscopy
Cell Population
Neural Cell
Article
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