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Journal / Immunology and Infection / Targeting dei tioli cisteina per in Vitro…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Targeting dei tioli cisteina per in Vitro site-specific glicosilazione delle proteine ricombinanti
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Journal (Immunology and Infection)
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Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
Journal (Immunology and Infection)
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Targeting dei tioli cisteina per in Vitro site-specific glicosilazione delle proteine ricombinanti

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Le analisi biochimiche e strutturali delle proteine glicosilate richiedono relativamente grandi quantità di campioni omogenei. Qui, presentiamo un efficiente metodo chimico per site-specific glicosilazione delle proteine ricombinanti purificate da batteri prendendo di mira i tioli reattivi di Cys.

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Immunologia problema 128 glicosilazione methanethiosulfonate modifica di cisteina del tiolo stromal interazione molecola-1 EFSAM spettroscopia di risonanza magnetica nucleare di soluzione spettrometria di massa
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