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Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
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Immunology and Infection
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JoVE Journal Immunology and Infection
Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

DOI:
10.3791/60393-v

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08:57 min

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October 06, 2019

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, ,
1Department of Biomedical Sciences,City University of Hong Kong

Chapters

  • 00:04Title
  • 01:00Stimulation of CAL-1 Cells
  • 02:23Extraction of Cellular Proteins
  • 03:39Analysis of IRF5 Dimerization by Native PAGE
  • 04:55Immunoblot Analysis of IRF5
  • 06:10Results: IRF5 Dimerization
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

A native Western blot method for analyzing endogenous interferon regulatory factor 5 dimerization in the CAL-1 plasmacytoid dendritic cell line is described. This protocol can be applied to other cell lines as well.

Tags

Keywords: Native Polyacrylamide Gel ElectrophoresisImmunoblot AnalysisEndogenous IRF5 DimerizationCAL-1 CellsR848 StimulationCell LysisProtein ExtractionNative PAGEWestern Blot

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