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JoVE Journal
Genetics

This content is Open Access.

Immunostaining for DNA Modifications
 

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Article DOI: 10.3791/56318-v 09:42 min September 7th, 2017
September 7th, 2017

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Summary

Newly discovered oxidized forms of 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) may represent distinct DNA modifications with unique functional roles. Here a semi-quantitative workflow for visualization of oxi-mCs' spatial distribution, signal intensity profiling and colocalization is described.

Tags

Keywords: DNA Modifications Immunostaining Confocal Microscopy Computational Analysis Epigenetics Nuclear Localization Signal Intensity 2.5D Visualization Image Processing Zen Blue Software
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