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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
JoVE Journal
Genetics
Author Produced
This content is Free Access.
JoVE Journal Genetics
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
DOI:

14:56 min

May 06, 2022

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Chapters

  • 00:03Introduction
  • 00:43Day 1: Bisulfite Treatment
  • 01:21Day 2: Amplification – Methylation Assay, Manual Protocol
  • 02:52Day 3: Hybridization – Methylation Assay, Manual Protocol
  • 06:39Day 4: Staining – Methylation Assay, Manual Protocol
  • 10:48Representative Results
  • 14:34Conclusion

Summary

Automatic Translation

The present study describes the workflow to manage DNA methylation data obtained by microarray technologies. The protocol demonstrates steps from sample preparation to data analysis. All procedures are described in detail, and the video shows the significant steps.

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