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Journal / Genetics / DNA 수정에 대 한 Immunostaining: 공초점 이미지의 계산…
JoVE Journal
Genetics

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JoVE Journal Genetics
DNA 수정에 대 한 Immunostaining
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DNA 수정에 대 한 Immunostaining: 공초점 이미지의 계산 분석

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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5-methylcytosine (oxi mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5 fC)의 산화 형태를 새로 발견 하 고 5-carboxylcytosine (5caC) 독특한 기능 역할을 가진 고유한 DNA 수정 나타낼 수 있습니다. 여기 oxi mCs의 공간 배급, 신호 강도 프로 파일링 및 colocalization의 시각화를 위한 반 정량 워크플로 설명 되어 있습니다.

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유전학 문제는 127 Epigenetics DNA oxi mCs N6-methyladenine Immunohistochemistry 젠 Confocal 현미경 검사 법 신호 정량 공간 배급
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