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Journal / Neuroscience / Isolatie en teelt van neurale progenitoren…
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JoVE Journal Neuroscience
Isolatie en teelt van neurale progenitoren gevolgd door chromatine-Immunoprecipitation van Histone 3 Lysine 79 Dimethylation Mark
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Isolatie en teelt van neurale progenitoren gevolgd door chromatine-Immunoprecipitation van Histone 3 Lysine 79 Dimethylation Mark

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Presenteren we een effectieve en reproduceerbare methode om te isoleren en cultuur van neurale voorlopercellen van embryonale en postnatale hersenweefsel voor chromatine immunoprecipitation (ChIP) van Histon 3 lysine 79 dimethylation (H3K79me2) - een Histon mark gelegen binnen de bolvormige domein van Histon 3.

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Neurowetenschappen kwestie 131 cerebellaire celcultuur corticale celcultuur schors cerebellaire ontwikkelen methylation van H3K79 DOT1L ChIP remming van de DOT1L
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